titin gene and protein functions in passive and active muscle pdf

Titin Gene And Protein Functions In Passive And Active Muscle Pdf

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Dilated cardiomyopathy DCM is a leading cause of heart failure, sudden cardiac death and heart transplant. TTN encodes titin, which is the largest protein in the body and is an essential component of the sarcomere.

Calcium-dependent titin–thin filament interactions in muscle: observations and theory

Gaps in our understanding of muscle mechanics demonstrate that the current model is incomplete. Increasingly, it appears that a role for titin in active muscle contraction might help to fill these gaps. While such a role for titin is increasingly accepted, the underlying molecular mechanisms remain unclear. Preliminary data support a role for Ig83, but other Ig domains in the N2A region may also be involved. Experimental observations demonstrate that these properties characterize wild type muscles, but not muscles from mdm mice with a small deletion in N2A titin, including part of Ig This is a preview of subscription content, access via your institution. Rent this article via DeepDyve.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. The giant protein titin is thought to be required for sarcomeric integrity in mature myocytes, but direct evidence for this hypothesis is limited. Here, we describe a mouse model in which Z-disc-anchored TTN is depleted in adult skeletal muscles. Inactivation of TTN causes sarcomere disassembly and Z-disc deformations, force impairment, myocyte de-stiffening, upregulation of TTN-binding mechanosensitive proteins and activation of protein quality-control pathways, concomitant with preferential loss of thick-filament proteins.

The giant protein titin is thought to play major roles in the assembly and function of muscle sarcomeres. Structural details, such as widths of Z- and M-lines and periodicities in the thick filaments, correlate with the substructure in the respective regions of the titin molecule. Sarcomere rest length, its operating range of lengths, and passive elastic properties are also directly controlled by the properties of titin. Here we review some recent titin data and discuss its implications for sarcomere architecture and elasticity. The complex but extremely ordered structure of the sarcomere is the elemental force-producing machinery of striated muscles. Recent studies of sarcomere assembly [ 1 , 2 ], protein turnover [ 1 , 3 ], and signalling cascades [ 4 , 5 ] provide new insights into the spectrum of intermolecular interactions that support sarcomere structure and function.

Roles of Titin in the Structure and Elasticity of the Sarcomere

Many studies have attempted to determine the associations between blood biomarkers and exercise-induced muscle damage. However, poor correlations between the changes in biomarker levels and the magnitude of muscle symptoms have been reported. Recent advances in proteomic tools offer a strategy for the comprehensive analysis of protein expression, which can be used to identify biomarkers. Here, we used a proteomic analysis to identify urinary proteins that appear in response to a calf-raise exercise, including repetitive eccentric muscle contractions, and found that a titin also known as connectin N-terminal fragment molecule appears in the urine after eccentric exercise. We measured the titin fragment in urine samples from nine individuals before and after eccentric exercise using a newly-established enzyme-linked immunosorbent assay and found that the titin fragment excretion rate increased 96 h after the exercise 5.

The role of titin in the modulation of cardiac function and its pathophysiological implications. Mailing address. Titin is a giant sarcomeric protein that extends from the Z-line to the M-line. Due to its location, it represents an important biomechanical sensor, which has a crucial role in the maintenance of the sarcomere structural integrity. Titin works as a "bidireactional spring" that regulates the sarcomeric length and performs adequate adjustments of passive tension whenever the length varies.


Titin Gene and Protein Functions in Passive and Active Muscle Abstract. The thin and thick filaments of muscle sarcomeres are interconnected by the giant protein.


Danielle Buck, John E. Smith, Charles S. J Gen Physiol 1 February ; 2 : — Titin is a molecular spring that determines the passive stiffness of muscle cells.

It comprises individually folded protein domains connected by unstructured peptide sequences. Titin is important in the contraction of striated muscle tissues. It connects the Z line to the M line in the sarcomere.

Introduction

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2 comments

Fleur A.

Findings suggest that titin stiffness is a principal regulator of the contractile behavior of striated muscle. Physiological or pathological changes to.

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Pamela S.

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